• Generation, characterization, and maintenance of induced pluripotent stem cells (iPSCs) from patients’ PBMCs
  • Differentiation of iPS cells into hepatocellular-like cells
  • Cell culture, primary cell culture and isolation of hematopoietic stem cells and immune cells
  • Conventional flow cytometry
  • Functional metabolic assays
  • Immunofluorescence microscopy, confocal microscopy and Light Sheet microscopy
  • Live cell imaging
  • Spatial Biology
  • RNA and Protein quantification (qPCR, ddPCR, immunoblotting)
  • Design of CRISPR editing platforms (CRISPR/Cas, base editing)
  • CRISPR safety (off targets sequencing)
  • Cloning of expression cassettes
  • Multiplex Immunoassay
  • Chromatin immunoprecipitation
  • Histochemistry and immunohistochemistry
  • Targeted Sequencing (Sanger/NGS)
  • Whole Genome Sequencing (WGS)
  • Diagnostic and follow-up testing for porphyrias